The campaigners took to the streets of the capital holding placards that read "Being a woman should not be a death sentence," "Patriarchy kills" while others featured names and photographs of the victims.
"Stop killing us," they chanted as they marched towards parliament, bringing traffic to a halt in Nairobi's central business district.
At least 16 women have been killed in Kenya this year, according to media reports, shining a spotlight on violence against women which the government has described as "rising."
In one of the cases that gained nationwide attention, a 26-year-old woman was killed on January 4 at a short-term rental apartment by a suspect who police say is part of an extortionist gang which targets women through dating sites.
Barely a fortnight later, a 20-year-old woman was strangled, dismembered and her remains stuffed into a plastic bag.
The gruesome killing triggered nationwide shock, including the chief government pathologist Johansen Oduor who said he had "never come across" such an incident over a two-decade forensic career.
Two men are in police custody over the case but are yet to be charged.
FILE - A human rights activist reacts as she attends a protest demanding an end to femicides in the country in Kenya's capital, Nairobi, January 27, 2024.
"Femicide is the most brutal manifestation of gender-based violence," Amnesty International's Kenya chapter said in a statement ahead of the march.
"It is unacceptable and must never be normalised," the rights group said, calling on authorities to expedite investigations and prosecution of the perpetrators.
- 'Time to stand up' -
At Saturday's protest, Terry Wangare, a communications officer, said it was "time for Kenya to stand up and make a decision."
"Nobody cares. If you complain, you are bad," the 32-year-old told AFP.
Student Faith Claire Wanjiru, 23, who was at her first protest, said she was "angry" and was not going to tolerate the violence.
"Taking someone's life should not be anyone's work," she said.
Organisers of the protest said other marches took place in 10 other regions including the lakeside city of Kisumu and the Indian Ocean port city of Mombasa.
More than 30 percent of women in Kenya experience physical violence and 13 percent experience some form of sexual violence, according to a government report released last year.
Human rights watchdogs are convinced that the number represents just a fraction of actual cases.
There were at least 152 cases of femicide in Kenya last year, according to non-profit Femicide Count, which keeps a tally of only reported incidents.
In 2022, some 725 women and girls were murdered in the East African country, according to a report from the UN Office on Drugs and Crime.