Apparently angry, and speaking at times with a raised voice, Prigozhin blamed Russia's Defense Minister Sergei Shoigu and Valery Gerasimov, the country's most senior soldier, of deliberately causing the arms shortages, which he said were causing heightened losses among Wagner troops fighting around the Ukrainian city of Bakhmut.
"There is simply direct opposition going on," Prigozhin said in a voice message posted on his Telegram channel. "This can be equated to high treason".
It is the second such message published by Prigozhin in two days. On Monday, he complained that unnamed officials were denying Wagner supplies out of personal animosity to him.
"The chief of the general staff and the defense minister are giving orders right and left not just not to give Wagner PMC ammunition, but not to help it with air transport," Prigozhin said.
Prigozhin has for months criticized senior commanders for what he has called their incompetence. Prigozhin has said that the defense ministry is trying to take credit for Wagner successes around the Donetsk region town of Bakhmut.
A onetime catering entrepreneur who once shunned the public spotlight, Prigozhin has assumed an more public role since the start of the war in Ukraine a year ago, with his Wagner Group spearheading Russia's months-long battle for the town of Bakhmut in Ukraine's Donetsk region.
The defense ministry could not be immediately contacted for comment.